Hieff™Adapter Ligation Module (Long-Read Sequencing) _ 13304ESHieffTM Adapter Ligation Module is a Motor Protein Ligation module specifically developed for the third generation sequencing platform. This kit features engineered and optimized enzymes and buffers, enabling the efficient ligation of sequencing adapters containing Motor Protein to both ends of the target DNA fragments. It can be used in conjunction with the HieffTM DNA Endprep & dA Tail Module (Cat#13302) and the Hieff NGSTM Barcode Ligation

and plasmid DNA (1 pg–1 μg) were used as templates for PCR amplification and gel electrophoresis analysis

CX3CL1 expression was found to be localized principally to neurons

Reconstituting to a concentration more than 100μg/mL is recommended

Arg46-Ile874

a 302 aa pro region and a 129 aa C-terminal mature peptide

Primer mix

and all the CV% were lower than 5% (Figure 2A)

upper body

RNA sequence analysis

thereby improving reaction efficiency

Baker’s yeast (diploid)

Add PMSF shortly before use to achieve a final concentration of 1 mM