Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) , His-Flag TagSpecifications Source Biotinylated Human CD40 Ligand TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N terminal. It contains Met113 Leu261.[Accession P29965] Molecular Weight The protein has a predicted MW of 52. 4 kDa, Due to glycosylation, the protein migrates to 55 60 kDa based on Tris Bis PAGE result. Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris Bis

The control group is the original protein

Recombinant Human IL-5

[43] Zhang X

the protein migrates to 46-48 kDa based on Tris-Bis PAGE result

Type of end product cDNA (First-Strand) PCR method RT-PCR Type of reagent Reverse Transcription Type of sample RNA Optimal reaction temperature 37℃ Reverse transcriptase M-MLV

55 x 10-9 M as determined in a SPR assay (Biacore 8K)

29 nM as determined in SPR assay

is an 8 kDa member of the CXC chemokine family

17 μM as determined in SPR assay

IL-13 exerts anti-inflammatory effects on monocytes and macrophages by inhibiting the expression of inflammatory cytokines such as IL-1β

48-well NTC detection was used in the specific detection of the product

In-Gel Detection Label or Dye Coomassie Product Type Specs Protein Gel Stain Reagent Target Molecule Protein